Molecular Profiling of Cystic Fluid for Improved Evaluation of Pancreatic Cysts

Dr. Sen and team will perform ancillary molecular analyses of cyst fluid samples from MCL patient cohorts that are being evaluated for genetic drivers and physical microenvironment together with measurable humoral immune response in cystic lesions. Molecular analyses of cyst fluid will include next-generation sequencing based mutation analyses of a panel of driver genes and profiling of differentially abundant microRNAs and metabolites associated with predisposition and development of pancreatic ductal adenocarcinoma (PDAC). The approach will develop an integrated biomarker profile, developed from the ancillary molecular analyses of cyst fluid, with significantly improved sensitivity and specificity in discriminating pre-malignant from indolent cystic lesions than currently possible with the available diagnostic methods.